- Linda Dexlin Mellby, PhD, VP R&D, Cellevate AB, Sweden
Viral vector manufacturing needs to ensure scalability while upholding high product yield, quality, and process robustness. Adherent cell cultures systems known for their ability to produce high yields of viral vectors are associated with scalability challenges. Cellevat3d™ nanofiber microcarriers, a novel microcarrier format, provides three-dimensional (3D) cell culturing of adherent cells readily scalable in stirred tank (suspension) bioreactor systems and offers promising avenues for scale up of adherent cell cultures. Data shows that HEK293T cells adhering to the nanofiber-based microcarriers form homogenous 3D cultures with high cell viability. In addition, the microcarriers large surface area and lightweight nature allow for achieving high cell densities and reduced shear forces in bioreactors. Importantly, transient transfection of adherent HEK293T cells growing on the nanofiber microcarriers shows high (>90%) transfection efficiency and volumetric productivity (1014 vg/L) of human adeno-associated virus serotype 2 (AAV2) vectors. These findings demonstrate the potential of the Cellevat3d™ nanofiber microcarriers as a scalable platform for adherent cell cultures in gene therapy applications, advancing bioprocessing strategies for enhanced yield, reduced time and costs leading to improved upstream bioprocessing productivity.
Session Objectives:
- Nanofiber-based microcarriers provide large surface area for high cell density cultures.
- High transfection efficiency and volumetric productivity are achieved for AAV vectors production.
- Nanofiber-based microcarriers is a scalable platform for adherent cell cultures in gene therapy applications.
- The platform shows the potential to improve the upstream bioprocessing productivity for viral vectors production
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